Journal: International Journal of Surgery (London, England)

Article Title: Targeting SIRT3 to regulate mitophagy-dependent ferroptosis for preventing glucocorticoid-induced osteoporosis

doi: 10.1097/JS9.0000000000002783

Figure Lengend Snippet: SIRT3 suppresses mitophagy-dependent ferroptosis, restoring the osteogenic capacity of MC3T3-E1 cells. (A) Machine learning (LASSO/SVM-RFE/Random Forest) identified GIOP-mitophagy associated genes. (B) SIRT3 expression correlated with GIOP progression. (C) qRT-PCR quantified PINK1/PARKIN mRNA in DEX-treated cells. (D-E) Western blot detected PINK1/PARKIN protein in DEX/DEX + SIRT3 groups. (F) IF visualized SIRT3 alterations (DEX group, 50 μm). (G) Multiplex IF showed GPX4/TFR/PINK1/PARKIN/SIRT3 changes (DEX/DEX + SIRT3, 50 μm). (H-I) Osteogenic capacity assessed by ALP/ARS staining. (J-K) Oxidative stress markers (MDA/GSH) measured. (L) qPCR analyzed PINK1/PARKIN/RUNX2/OPG/SIRT3 expression. Experimental design: n =3 biological replicates (technical triplicates). Data: mean ± SD. Statistics: one-way ANOVA. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

Article Snippet: A cohort of 40 male Sprague-Dawley rats (8-week-old, SPF-grade) were randomly allocated into four groups (n = 10/group): Control: PBS vehicle Model: GIOP Mdivi-1: GIOP + 15 mg/kg Mdivi-1 (MedChemExpress, HY-15 886) i.p. every 48 h [ ] SIRT3 agonist: GIOP + 200 mg/kg nicotinamide riboside (MedChemExpress, HY-15 452) p.o. daily [ ] .

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Multiplex Assay, Staining

Journal: International Journal of Surgery (London, England)

Article Title: Targeting SIRT3 to regulate mitophagy-dependent ferroptosis for preventing glucocorticoid-induced osteoporosis

doi: 10.1097/JS9.0000000000002783

Figure Lengend Snippet: SIRT3 prevents GIOP by regulating mitophagy-dependent ferroptosis (A) Tibial sections were co-stained for FTH/GPX4 and RUNX2/OPG (scale bar = 500 μm). (B) Intracellular ROS levels were visualized by fluorescence staining (scale bar = 1000 μm). (C) Mitophagy markers PINK1/PARKIN were co-localized by IF (scale bar = 100 μm). (D) Micro-CT reconstructed 2D trabecular architecture. (E) Histomorphometric analysis of bone structure (HE staining, scale bar = 200 μm). Experimental design: n =5 biological replicates/group. Data presented as mean ± SD. Statistical analysis: two-tailed Student’s t-test. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)

Article Snippet: A cohort of 40 male Sprague-Dawley rats (8-week-old, SPF-grade) were randomly allocated into four groups (n = 10/group): Control: PBS vehicle Model: GIOP Mdivi-1: GIOP + 15 mg/kg Mdivi-1 (MedChemExpress, HY-15 886) i.p. every 48 h [ ] SIRT3 agonist: GIOP + 200 mg/kg nicotinamide riboside (MedChemExpress, HY-15 452) p.o. daily [ ] .

Techniques: Staining, Fluorescence, Micro-CT, Two Tailed Test

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 1. Characteristics of changes in serum levels of Sirt3 in patients with ICU. (a) Serum Sirt3 levels were measured in sepsis group, septic shock group, and ICU control group. (b) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with sepsis on day 1. (c) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with septic shock on day 1. (d) Serum Sirt3 levels were measured in sepsis group and septic shock group after re-diagnosed on D3 and D5. P < 0.05 were considered statistically significant. *denotes P < 0.05, **denotes P < 0.01, ****denotes P < 0.0001, “ns” means no significance.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: Control

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 2. Correlation between Sirt3 and clinical indicators in ICU patients. (a) Correlation between serum Sirt3 and SOFA score in sepsis group, septic shock group, and ICU control group. (b) Correlation between serum Sirt3 and lactate in sepsis group, septic shock group, and ICU control group. (c) Correlation between serum Sirt3 and ScvO2 in sepsis group and septic shock group. (d) Correlation between serum Sirt3 and PCT in sepsis group and septic shock group. P < 0.05 were considered statistically significant. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: Control

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 3. The ROC curves of indicators for the diagnosis of sepsis and the early detection of sepsis from septic shock. (a) The ROC curves of indicators for the diagnosis of sepsis (PCT, Sirt3, SOFA). (b) The ROC curves of indicators for the early detection of septic shock (lactate, Scvo2, Sirt3, lactate + Sirt3, Lactate + ScvO2, P(V-A)CO2). Calculate the predicted values for lactate + Sirt3 and lactate + ScvO2 by binary logistic regression and then calculate the ROC curve. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin, P(V-A)CO2 central venous-to-arterial blood carbon dioxide partial pressure.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: Biomarker Discovery

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 4. The ROC curves of predicting for 28-day survival and Kaplan–Meier estimator analysis of Sirt3 for 28 day survival. (a) The ROC curves of Sirt3 for the predicting 28-day mortality in patients with sepsis. P < 0.05 were considered statistically significant (b) Kaplan–Meier estimator analysis of Sirt3 for 28-day survival. The cut off value of Sirt3 for the estimated 28-day mortality of patients with sepsis was 10.07 pg/ml.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: